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Clinical Chemistry 51: 2159-2162, 2005; 10.1373/clinchem.2005.050203
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(Clinical Chemistry. 2005;51:2159-2162.)
© 2005 American Association for Clinical Chemistry, Inc.

Technical Briefs

Altered Distribution of Transferrin Isoforms According to Serum Storage Conditions

Brice M.R. Appenzellera and Robert Wennig

Centre de Recherche Public–Santé, Laboratoire National de Santé, Toxicologie, Université du Luxembourg, 162A, Ave de la Faïencerie, L-1511 Luxembourg;

aauthor for correspondence: fax 352-22-13-31, e-mail appenzel@cu.lu

The first 300 words of the full text of this article appear below.

Measurements of carbohydrate-deficient transferrin (CDT) are used to detect alcohol abuse (1)(2)(3)(4)(5)(6). Various patient factors(7)(8)(9)(10)(11) and comigration of analytes with transferrin (Tf) isoforms(12)(13) can affect the results of the measurements. Recent studies have questioned the influence of serum and blood storage conditions on CDT and yielded varying (and sometimes contradictory) findings(14)(15)(16)(17). Moreover, the stability of CDT may vary among serum samples(14)(16).

We studied CDT and Tf isoforms in serum specimens stored at 25 °C, 4 °C, and –20 °C. We used 100 discarded serum samples; 8 were from alcohol abusers in treatment (4 with increased disialo-Tf and the presence of asialo-Tf; 4 with increased disialo-Tf but without asialo-Tf), and 6 were from moderate drinkers (CDT within reference values). For sera from alcohol abusers, the alcohol abuse diagnosis was based on psychological evaluation; CDT, {gamma}-glutamyltransferase, and transaminase (aspartate aminotransferase and alanine aminotransferase) concentrations; and erythrocyte mean corpuscular volume. Each of the 14 specimens was divided into 3 samples and stored in glass vials (4 mL; Supelco 27137) at room temperature (25 °C in the dark), in a refrigerator (4 °C), or in a freezer (–20 °C). Samples were then analyzed periodically for 28–81 days [mean (SD), 51.6 (14.7) days]. The main reason for discontinuing follow-up was the appearance of solid particles in the serum. For frozen samples (–20 °C), the time between thawing and refreezing was <5 min. Serum Tf was analyzed (13) by use of capillary electrophoresis (P/ACE 5500) and a reagent set (CEofix; ANALIS). Electropherograms displayed all major Tf isoforms: asialo-Tf (when present), disialo-Tf, trisialo-Tf, tetrasialo-Tf, pentasialo-Tf, and hexasialo-Tf. . . . [Full Text of this Article]






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