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Clinical Chemistry 51: 776-778, 2005. First published February 3, 2005; 10.1373/clinchem.2004.047142
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(Clinical Chemistry. 2005;51:776-778.)
© 2005 American Association for Clinical Chemistry, Inc.

Technical Briefs

Effects of Hemoglobin C and S Traits on Glycohemoglobin Measurements by Eleven Methods

William L. Roberts1,a, Sekineh Safar-Pour2, Barun K. De3, Curt L. Rohlfing4, Cas W. Weykamp5 and Randie R. Little4

1 Department of Pathology, ARUP Institute for Clinical & Experimental Pathology, University of Utah, Salt Lake City, UT;2 ARUP Laboratories, Salt Lake City, UT;3 Department of Pathology, University of Arizona, Tucson, AZ;4 Departments of Pathology & Anatomical Sciences and Child Health, University of Missouri-Columbia School of Medicine, Columbia, MO;5 Queen Beatrix Hospital, Winterswijk, The Netherlands

aaddress correspondence to this author at: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108; fax 801-584-5207, e-mail william.roberts@aruplab.com

The first 20% of the full text of this article appears below.

Patients with diabetes mellitus routinely have glycohemoglobin (GHb) testing performed to monitor glycemic control and assess risk for developing complications of their disease (1). The accuracy of several GHb methods can be adversely affected by the presence of hemoglobin (Hb) C or S trait (2)(3)(4)(5)(6). It has been estimated that there are at least 200 000 Americans with diabetes mellitus who also have either Hb C or S trait (6). We have recently shown that the presence of Hb C or S trait does not affect the accuracy of GHb measurements made by the CLC 330 boronate affinity HPLC method (7). We therefore evaluated the effects of Hb C and S traits on 11 commercial GHb methods, using the CLC 330 assay as the comparison method.

Whole blood samples from individuals homozygous for Hb A (n = 73) and heterozygous for Hb C or S (n = 46 and 76, respectively) were collected in EDTA-containing tubes. After routine clinical testing had been completed, Hb variants were identified by inspection of chromatograms obtained with a VARIANT analyzer (Bio-Rad Laboratories) and the Beta Thal Short program run according to the manufacturer’s instructions. Aliquots of these samples that had 4–14% Hb A1c were stored at 2–8 °C and analyzed within 10 days of collection except for aliquots for the HA8160 and HA8160 Beta Thal (BT) methods, which were shipped on dry ice and stored frozen until analysis. Not all samples were analyzed by each analytic method. . . . [Full Text of this Article]




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