Clinical Chemistry
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Clinical Chemistry 52: 152-153, 2006; 10.1373/clinchem.2005.057547
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(Clinical Chemistry. 2006;52:152-153.)
© 2006 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Effect of Addition of Hemolysate on Urine and Cerebrospinal Fluid Assays for Protein

Fatma Meriç Yilmaz and Dogan Yücela

Ministry of HealthAnkara Hospital Clinical Biochemistry Laboratory Ankara 06340, Turkey

aAuthor for correspondence. Fax 90-312-3621857; e-mail doyucel@yahoo.com.

The first 20% of the full text of this article appears below.


To the Editor:

The presence of hemolyzed erythrocytes is common in urine and spinal fluid samples, and the released cellular contents, primarily hemoglobin (Hb), may affect protein measurements. Hematuria may be seen with or without the presence of other proteins in urine. Similarly, in hemorrhagic stroke, erythrocytes in the cerebrospinal fluid (CSF) begin to lyse within 2–3 h (1). Hb can be measured by protein assays, but may produce spectral and chemical interferences.

We investigated the effect of hemolysis on Pyrogallol Red (PYR) (2), benzethonium chloride (BTC)(3), and benzalkonium chloride (BC) (4) methods for measurement of protein. We prepared 3 urine and 3 CSF pools with different protein concentrations and added hemolysates . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


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E. J Lamb, F. MacKenzie, and P. E Stevens
How should proteinuria be detected and measured?
Ann Clin Biochem, May 1, 2009; 46(3): 205 - 217.
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J. L. Carter, C. R. V. Tomson, P. E. Stevens, and E. J. Lamb
Does urinary tract infection cause proteinuria or microalbuminuria? A systematic review
Nephrol. Dial. Transplant., November 1, 2006; 21(11): 3031 - 3037.
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