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Clinical Chemistry 52: 2127-2128, 2006; 10.1373/clinchem.2006.074567
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(Clinical Chemistry. 2006;52:2127-2128.)
© 2006 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Reliability of Thrombin Generation Assay on Frozen-Thawed Platelet-Rich Plasma: A Reply

Nathalie Hézard, Marie-Geneviève Remy, Bernadette Florent and Philippe Nguyena

Laboratoire d’Hématologie, CHU Robert Debré, Reims, France

aAddress correspondence to this author at: Laboratoire d’Hématologie, CHU Robert Debré, 51092 Reims Cedex, France. Fax 33-3-26-78-81-71; e-mail pnguyen@chu-reims.fr.

The first 20% of the full text of this article appears below.


To the Editor:

We read with interest the comments of Lippi et al (1) who questioned the reproducibility and difficulty of standardization when we (2) used platelet-rich plasma (PRP) in the thrombin generation assay (TGA). We evaluated the use of TGA to identify patients needing further specific thrombophilic risk factor testing (2). For this, we used frozen-thawed (ft-PRP). PRP was obtained by centrifugation (190g for 12 min), adjusted to 150 x 109/L, and immediately frozen at –80 °C. Samples were immediately thawed before the assay. TGA was triggered by the addition of 0.5 pmol/L of recombinant human . . . [Full Text of this Article]






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