Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 52: 1446-1448, 2006; 10.1373/clinchem.2006.069971
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow 069971.Supplemental Data
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, Q.
Right arrow Articles by Li, Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, Q.
Right arrow Articles by Li, Q.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 2006;52:1446-1448.)
© 2006 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Preparation of a Chimeric Armored RNA as a Versatile Calibrator for Multiple Virus Assays

Qiuying Huang, Yangjian Cheng, Qiwei Guo and Qingge Lia

Molecular Diagnostics Laboratory, Department of Biomedical Sciences, and the, Key Laboratory of Cell Biology, and, Tumor Cell Engineering, of the Ministration of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China

aAddress correspondence to this author at: Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China. Fax 86-592-2187363; e-mail qgli@xmu.edu.cn.

The first 20% of the full text of this article appears below.


To the Editor:

As with all diagnostic techniques, molecular testing requires careful quality control (1)(2)(3). In detection of RNA viruses, which are often present at low concentrations and are prone to degradation, stringent monitoring is needed for all aspects of assay performance, including virus lysis, RNA isolation, reverse transcription, amplification, and detection steps. Among many proposed RNA control preparations (4)(5), armored RNA is currently the most suitable for clinical applications as it carries the viral RNA target of interest in a form that is ribonuclease-resistant, noninfectious, and stable after prolonged incubation in clinical matrices, and the preparations are substantially less expensive to manufacture than virusinfected plasma (6)(7)(8). Thus, armored RNA has been applied as a positive control for a variety of RNA viruses (9).

Because most . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Microbiol.Home page
Y. Wei, C. Yang, B. Wei, J. Huang, L. Wang, S. Meng, R. Zhang, and J. Li
RNase-Resistant Virus-Like Particles Containing Long Chimeric RNA Sequences Produced by Two-Plasmid Coexpression System
J. Clin. Microbiol., May 1, 2008; 46(5): 1734 - 1740.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
X.-F. Yu, J.-C. Pan, R. Ye, H.-Q. Xiang, Y. Kou, and Z.-C. Huang
Preparation of Armored RNA as a Control for Multiplex Real-Time Reverse Transcription-PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus
J. Clin. Microbiol., March 1, 2008; 46(3): 837 - 841.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Association for Clinical Chemistry.