Clinical Chemistry
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Clinical Chemistry 54: 446-447, 2008; 10.1373/clinchem.2007.093914
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(Clinical Chemistry. 2008;54:446-447.)
© 2008 American Association for Clinical Chemistry, Inc.

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Phase Switching SPE for Faster 1,25-dihydroxyvitamin D Analysis

Bruce W. Hollis1

1 Department of Pediatrics and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC.

aAddress correspondence to the author at: Departments of Pediatrics, Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425. E-mail hollisb@musc.edu.

The first 20% of the full text of this article appears below.

Feature Article: Hollis BW. Assay of circulating 1,25-dihydroxyvitamin D involving a novel single-cartridge extraction and purification procedure. Clin Chem 1986;32:2060–63.1

This paper described a simplified method for extracting and purifying 1,25(OH)2D from serum or plasma before quantification by radioreceptor assay (RA). Following precipitation of plasma proteins with acetonitrile and centrifugation, the supernatant was removed, combined with pH 10.5 buffer, and applied to a C18 nonendcapped solid-phase extraction cartridge. The cartridge was washed with various solvent combinations, and the 1,25(OH)2D-containing fraction was selectively eluted. After evaporation the residue was dissolved in ethanol and analyzed by RA for 1,25(OH)2D content.

In 1982 I was awarded an NIH RO1 grant to study the vitamin D requirement of newborn infants. One of the specific aims, measuring the circulating 1,25(OH)2D in our study participants, . . . [Full Text of this Article]






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