Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 54: 446-447, 2008; 10.1373/clinchem.2007.093914
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hollis, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hollis, B. W.
Related Collections
Right arrow Citation Classics
(Clinical Chemistry. 2008;54:446-447.)
© 2008 American Association for Clinical Chemistry, Inc.

Citation Classics

Phase Switching SPE for Faster 1,25-dihydroxyvitamin D Analysis

Bruce W. Hollis1

1 Department of Pediatrics and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC.

aAddress correspondence to the author at: Departments of Pediatrics, Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425. E-mail hollisb@musc.edu.

The first 20% of the full text of this article appears below.

Feature Article: Hollis BW. Assay of circulating 1,25-dihydroxyvitamin D involving a novel single-cartridge extraction and purification procedure. Clin Chem 1986;32:2060–63.1

This paper described a simplified method for extracting and purifying 1,25(OH)2D from serum or plasma before quantification by radioreceptor assay (RA). Following precipitation of plasma proteins with acetonitrile and centrifugation, the supernatant was removed, combined with pH 10.5 buffer, and applied to a C18 nonendcapped solid-phase extraction cartridge. The cartridge was washed with various solvent combinations, and the 1,25(OH)2D-containing fraction was selectively eluted. After evaporation the residue was dissolved in ethanol and analyzed by RA for 1,25(OH)2D content.

In 1982 I was awarded an NIH RO1 grant to study the vitamin D requirement of newborn infants. One of the specific aims, measuring the circulating 1,25(OH)2D in our study participants, . . . [Full Text of this Article]






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the American Association for Clinical Chemistry.