Clinical Chemistry
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Clinical Chemistry 55: 1436-1438, 2009. First published May 7, 2009; 10.1373/clinchem.2009.125609
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(Clinical Chemistry. 2009;55:1436-1438.)
© 2009 American Association for Clinical Chemistry, Inc.

Letters to the Editor

A Simple Homemade Apparatus for Real-Time Visualization of Nucleic Acid Electrophoresis That Saves Time, Optimizes Separation, and Reduces Chemical Risk

Giovanni Destro Bisol2,3,a and Cristiano Ariano2

2 Università di Roma "La Sapienza", Dipartimento di Biologia Animale e dell’Uomo, Laboratorio di Antropologia Molecolare, Roma, Italy, 3 Istituto Italiano di Antropologia, Roma, Italy

aAddress correspondence to this author at:, Università di Roma "La Sapienza", Dipartimento di Biologia Animale e dell’Uomo, P.le A. Moro 5, 00185 Roma, Italy, Fax 00390649912771, E-mail giovanni.destrobisol@uniroma1.it

The first 20% of the full text of this article appears below.


To the Editor:

Agarose slab gel electrophoresis continues to provide a low-cost alternative and a valuable complement to more sophisticated techniques for estimating the size of DNA molecules after restriction enzyme digestion, for evaluating PCR products, and for separating restricted genomic DNA or RNA before Southern or northern blot analysis (1)(2).

With traditional apparatus, the electrophoresis run must be interrupted before the agarose gel can be moved onto a UV transilluminator (wavelength approximately 300–360 nm), which is connected to a conventional or digital camera and placed in a dark environment for best results. This procedure is repeated until a satisfactory separation is achieved.

We have devised a simple, easily reproducible device (Fig. 1 ) that contains the following main components: (a) an electrophoresis chamber; (b) 2 UV light–emitting lamps (302 nm), which are placed below the plastic plate holding the gel; and (c) a commercial Web camera connected to a personal computer. The device can be opened to pour or remove a gel and to fill . . . [Full Text of this Article]






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Copyright © 2009 by the American Association for Clinical Chemistry.