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Clinical Chemistry 18: 499-502, 1972;
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Clinical Chemistry, Vol 18, 499-502, Copyright © 1972 by the American Association for Clinical Chemistry

Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative Ultracentrifuge

William T. Friedewald 1, Robert I. Levy 1, and Donald S. Fredrickson 1

1 Biometrics Research Branch (W.T.F.) and the Molecular Disease Branch (R.I.L. and D.S.F.), National Heart and Lung Institute, 9000 Rockville Pike, Bethesda, Md. 20014.

A method for estimating the cholesterol content of the serum low-density lipoprotein fraction (Sf0-20) is presented. The method involves measurements of fasting plasma total cholesterol, triglyceride, and high-density lipoprotein cholesterol concentrations, none of which requires the use of the preparative ultracentrifuge. Comparison of this suggested procedure with the more direct procedure, in which the ultracentrifuge is used, yielded correlation coefficients of .94 to .99, depending on the patient population compared.


Key Words: hyperlipoproteinemia classification • determination of plasma total cholesterol, triglyceride, high-density lipoprotein cholesterol • beta lipoproteins

Submitted on February 29, 1972
Accepted on March 13, 1972




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