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Multiplexed Genetic Analysis Using an Expanded Genetic Alphabet

¹ Eragen Biosciences, Inc., Madison, WI.
² Wisconsin State Laboratory of Hygiene, Madison, WI.
³ Department of Pediatrics, University of Wisconsin, Madison, WI.
⁴ Department of Chemistry, University of Florida, Gainesville, FL.

^aAddress correspondence to this author at: Eragen Biosciences, Inc., 918 Deming Way, Madison, WI 53717. Fax 608-662-9004; e-mail jprudent{at}eragen.com.

Background: All states require some kind of testing for newborns, but the policies are far from standardized. In some states, newborn screening may include genetic tests for a wide range of targets, but the costs and complexities of the newer genetic tests inhibit expansion of newborn screening. We describe the development and technical evaluation of a multiplex platform that may foster increased newborn genetic screening.

Methods: MultiCode® PLx involves three major steps: PCR, target-specific extension, and liquid chip decoding. Each step is performed in the same reaction vessel, and the test is completed in 3 h. For site-specific labeling and room-temperature decoding, we use an additional base pair constructed from isoguanosine and isocytidine. We used the method to test for mutations within the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The developed test was performed manually and by automated liquid handling. Initially, 225 samples with a range of genotypes were tested retrospectively with the method. A prospective study used samples from >400 newborns.

Results: In the retrospective study, 99.1% of samples were correctly genotyped with no incorrect calls made. In the perspective study, 95% of the samples were correctly genotyped for all targets, and there were no incorrect calls.

Conclusions: The unique genetic multiplexing platform was successfully able to test for 31 targets within the CFTR gene and provides accurate genotype assignments in a clinical setting.

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