HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: clinchem.2003.026138v1 50/5/867    most recent Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (46) Citing Articles via Google Scholar Google Scholar Articles by Wu, A. H.B. Articles by Ghani, F. Search for Related Content PubMed PubMed Citation Articles by Wu, A. H.B. Articles by Ghani, F. Related Collections Proteomics and Protein Markers Lipids, Lipoproteins, and Cardiovascular Risk Factors

Analytical and Clinical Evaluation of the Bayer ADVIA Centaur Automated B-Type Natriuretic Peptide Assay in Patients with Heart Failure: A Multisite Study

¹ Department of Pathology and Laboratory Medicine, Hartford Hospital, 80 Seymour St., Hartford, CT 06102.² Departments of Medicine and Pathology, Columbia Presbyterian Medical Center, New York, NY.³ Department of Internal Medicine/Division of Cardiology, University of Innsbruck, Innsbruck, Austria.⁴ Uppsala Clinical Research Center and⁵ Department of Cardiology, Cardiothoracic Center, Uppsala University Hospital, Uppsala, Sweden.⁶ Pathology Department, Henry Ford Hospital, Detroit, MI.⁷ Department of Pathology, University of Virginia, Charlottesville, VA.⁸ Centre for Research and Evaluation in Diagnostics, CRED, Sherbrooke, Quebec, Canada.⁹ Bayer Healthcare LLC, Diagnostics Division, Tarrytown, NY.

^aAuthor for correspondence. Fax 860-545-3733; e-mail awu{at}harthosp.org.

Background: B-Type natriuretic peptide (BNP) is released from the left ventricle of the heart into the circulation in response to ventricular stretching and volume overload. Increased BNP concentrations are associated with heart failure (HF).

Methods: We evaluated the analytical and clinical performance of the Bayer ADVIA Centaur® BNP assay. Studies included precision, analytical correlation (against the Shionogi ShionoRIA^TM and Biosite Triage® BNP assays), BNP results for blood collected in plastic tubes containing EDTA vs other collection tubes, high-dose hook effect, detection limits, and interferences. The clinical performance was tested on 2243 blood samples collected from 983 apparently healthy individuals, 538 patients with chronic disease but without HF (renal insufficiency, chronic obstructive pulmonary disease, diabetes, and hypertension), and 722 patients with HF (New York Heart Association classes I-IV).

Results: The ADVIA Centaur assay had total imprecision (CV) of 3.4%, 2.9%, and 2.4% at BNP concentrations of 48, 461, and 1768 ng/L, respectively. The Passing–Bablok correlations to the ShionoRIA and Triage were as follows: ADVIA Centaur = 1.11(ShionoRIA) – 1.19 ng/L (r = 0.98); ADVIA Centaur = 0.78(Triage) + 5.89 ng/L (r = 0.92), respectively. Of the different blood collection tubes, only EDTA plastic tubes (with and without the barrier gel) were acceptable. The lower detection limit was 0.5 ng/L, and there were no interferences from common analytes, other neuropeptides, or unusual antibodies. BNP exhibited different reference intervals according to age and gender. BNP concentrations increased progressively as the severity of HF increased.

Conclusions: The ADVIA Centaur is the first commercially available BNP assay for use on an automated immunochemistry platform. This assay has good analytical and clinical performance characteristics for diagnosing HF.

The following articles in journals at HighWire Press have cited this article:

				N. N. Tamm, K. R. Seferian, A. G. Semenov, K. S. Mukharyamova, E. V. Koshkina, M. I. Krasnoselsky, A. B. Postnikov, D. V. Serebryanaya, F. S. Apple, M. M. Murakami, et al. Novel Immunoassay for Quantification of Brain Natriuretic Peptide and Its Precursor in Human Blood Clin. Chem., September 1, 2008; 54(9): 1511 - 1518. [Abstract] [Full Text] [PDF]

				M. Damgaard, J. P. Goetze, P. Norsk, and N. Gadsboll Altered sodium intake affects plasma concentrations of BNP but not proBNP in healthy individuals and patients with compensated heart failure Eur. Heart J., November 2, 2007; 28(22): 2726 - 2731. [Abstract] [Full Text] [PDF]

				B. H. Cuthbertson, A. R. Amiri, B. L. Croal, S. Rajagopalan, O. Alozairi, J. Brittenden, and G. S. Hillis Utility of B-type natriuretic peptide in predicting perioperative cardiac events in patients undergoing major non-cardiac surgery Br. J. Anaesth., August 1, 2007; 99(2): 170 - 176. [Abstract] [Full Text] [PDF]

				R. Carrillo-Jimenez, S. Borzak, and C. H. Hennekens Brain Natriuretic Peptide: Clinical and Research Challenges Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2007; 12(2): 85 - 88. [Abstract] [PDF]

				R. Sakhuja, S. Green, E. M. Oestreicher, P. M. Sluss, E. Lee-Lewandrowski, K. B. Lewandrowski, and J. L. Januzzi Jr. Amino-Terminal Pro-Brain Natriuretic Peptide, Brain Natriuretic Peptide, and Troponin T for Prediction of Mortality in Acute Heart Failure Clin. Chem., March 1, 2007; 53(3): 412 - 420. [Abstract] [Full Text] [PDF]

				D. A. Morrow, J. A. de Lemos, M. S. Sabatine, S. D. Wiviott, M. A. Blazing, A. Shui, N. Rifai, R. M. Califf, and E. Braunwald Clinical Relevance of C-Reactive Protein During Follow-Up of Patients With Acute Coronary Syndromes in the Aggrastat-to-Zocor Trial Circulation, July 25, 2006; 114(4): 281 - 288. [Abstract] [Full Text] [PDF]

				P. Kupchak, A. H.B. Wu, F. Ghani, L. K. Newby, E. M. Ohman, and R. H. Christenson Influence of Imprecision on ROC Curve Analysis for Cardiac Markers Clin. Chem., April 1, 2006; 52(4): 752 - 753. [Abstract] [Full Text] [PDF]

				D. A. Morrow, J. A. de Lemos, M. A. Blazing, M. S. Sabatine, S. A. Murphy, P. Jarolim, H. D. White, K. A. A. Fox, R. M. Califf, E. Braunwald, et al. Prognostic Value of Serial B-Type Natriuretic Peptide Testing During Follow-up of Patients With Unstable Coronary Artery Disease JAMA, December 14, 2005; 294(22): 2866 - 2871. [Abstract] [Full Text] [PDF]

				A. M. Hawkridge, D. M. Heublein, H. R. Bergen III, A. Cataliotti, J. C. Burnett Jr., and D. C. Muddiman Quantitative mass spectral evidence for the absence of circulating brain natriuretic peptide (BNP-32) in severe human heart failure PNAS, November 29, 2005; 102(48): 17442 - 17447. [Abstract] [Full Text] [PDF]

				A. Clerico, C. Prontera, M. Emdin, C. Passino, S. Storti, R. Poletti, L. Zyw, and G. C. Zucchelli Analytical Performance and Diagnostic Accuracy of Immunometric Assays for the Measurement of Plasma B-Type Natriuretic Peptide (BNP) and N-Terminal proBNP Clin. Chem., February 1, 2005; 51(2): 445 - 447. [Full Text] [PDF]

				A. Hammerer-Lercher, W. Ludwig, G. Falkensammer, S. Muller, E. Neubauer, B. Puschendorf, O. Pachinger, and J. Mair Natriuretic Peptides as Markers of Mild Forms of Left Ventricular Dysfunction: Effects of Assays on Diagnostic Performance of Markers Clin. Chem., July 1, 2004; 50(7): 1174 - 1183. [Abstract] [Full Text] [PDF]