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This Article Full Text Full Text (PDF) Data Supplements All Versions of this Article: clinchem.2005.052324v1 51/12/2236    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (37) Citing Articles via Google Scholar Google Scholar Articles by Cheng, A.-J. Articles by Chen, I-H. Search for Related Content PubMed PubMed Citation Articles by Cheng, A.-J. Articles by Chen, I-H. Related Collections Proteomics and Protein Markers

Oral Cancer Plasma Tumor Marker Identified with Bead-Based Affinity-Fractionated Proteomic Technology

Departments of¹ Medical Biotechnology and ² Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, Taiwan.
³ Department of Radiation Oncology, ⁴ Division of Hematology/Oncology, Department of Internal Oncology, and ⁵ Department of Otorhinolaryngology, Section of Head and Neck Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

^aAddress correspondence to this author at: Graduate School of Medical Biotechnology, Chang Gung University, 259 Wen-Hwa 1st Road, Taoyuan 333, Taiwan. Fax 886-3-2118247; e-mail ajchen{at}mail.cgu.edu.tw.

Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity-fractionated proteomic method to discover a novel plasma marker for oral cancer.

Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis^TM and ClinProt^TM bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18–39). For identification, affinity bead–purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database.

Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were <4% and 7%, respectively. Six markers that differentiated between cancer and control spectra were found, with mean (SD) molecular masses of 2664 (1), 2850 (1), 3250 (1), 7735 (2), 7927 (2), and 9240 (2) Da. The 2664-Da marker, identified as a fragment of the fibrinogen -chain, had the highest sensitivity (100%) and specificity (97%) for cancer.

Conclusion: The high specificity and sensitivity of the fibrinogen -chain fragment suggest that it may be a clinical useful tumor marker.

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