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Endocrinology and Metabolism |
1 ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT.
Departments of2
Pathology and 4
Medicine, University of Utah, Salt Lake City, UT.
3 Department of Pathology, VA San Diego Healthcare System, University of California, San Diego, CA.
aAddress correspondence to this author at: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108. Fax 801-584-5207; e-mail kushnmm{at}aruplab.com.
Background: Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagnosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and children.
Methods: Te was extracted with methyl tert-butyl ether from 100 µL of serum or plasma, derivatized to form an oxime, and reextracted by solid-phase extraction. Instrumental analysis was performed on an API 4000 HPLC tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode. The MRM transitions (m/z) were 304
124 and 304112 for Te and 307124 and 307112 for d3-Te.
Results: Within- and between-run CVs were <12% and 7.9%, respectively. The limit of quantification was 0.0346 nmol/L (1 ng/dL). Reference intervals for sex hormone–binding globulin and total, free, and bioavailable Te were established for children of Tanner stages 1 through 5 and adult males and females.
Conclusions: The sensitivity and specificity of the method are adequate for analysis of Te in samples from women and children. The method requires small sample volumes, has adequate precision, and is not subject to interferences.
The following articles in journals at HighWire Press have cited this article:
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  J. Jonklaas, N. Kahric-Janicic, O. P. Soldin, and S. J. Soldin Correlations of Free Thyroid Hormones Measured by Tandem Mass Spectrometry and Immunoassay with Thyroid-Stimulating Hormone across 4 Patient Populations Clin. Chem., July 1, 2009; 55(7): 1380 - 1388. [Abstract] [Full Text] [PDF]
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M. M. Kushnir, T. Naessen, D. Kirilovas, A. Chaika, J. Nosenko, I. Mogilevkina, A. L. Rockwood, K. Carlstrom, and J. Bergquist Steroid Profiles in Ovarian Follicular Fluid from Regularly Menstruating Women and Women after Ovarian Stimulation Clin. Chem., March 1, 2009; 55(3): 519 - 526. [Abstract] [Full Text] [PDF]
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S. Shiraishi, P. W. N. Lee, A. Leung, V. H. H. Goh, R. S. Swerdloff, and C. Wang Simultaneous Measurement of Serum Testosterone and Dihydrotestosterone by Liquid Chromatography-Tandem Mass Spectrometry Clin. Chem., November 1, 2008; 54(11): 1855 - 1863. [Abstract] [Full Text] [PDF]
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L. M. Thienpont, K. Van Uytfanghe, S. Blincko, C. S. Ramsay, H. Xie, R. C. Doss, B. G. Keevil, L. J. Owen, A. L. Rockwood, M. M. Kushnir, et al. State-of-the-Art of Serum Testosterone Measurement by Isotope Dilution-Liquid Chromatography- Tandem Mass Spectrometry Clin. Chem., August 1, 2008; 54(8): 1290 - 1297. [Abstract] [Full Text] [PDF]
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 C. Meier, T. V. Nguyen, D. J. Handelsman, C. Schindler, M. M. Kushnir, A. L. Rockwood, A. W. Meikle, J. R. Center, J. A. Eisman, and M. J. Seibel Endogenous Sex Hormones and Incident Fracture Risk in Older Men: The Dubbo Osteoporosis Epidemiology Study Arch Intern Med, January 14, 2008; 168(1): 47 - 54. [Abstract] [Full Text] [PDF]
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T. M. Annesley Methanol-Associated Matrix Effects in Electrospray Ionization Tandem Mass Spectrometry Clin. Chem., October 1, 2007; 53(10): 1827 - 1834. [Abstract] [Full Text] [PDF]
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 W. Rosner, R. J. Auchus, R. Azziz, P. M. Sluss, and H. Raff Utility, Limitations, and Pitfalls in Measuring Testosterone: An Endocrine Society Position Statement J. Clin. Endocrinol. Metab., February 1, 2007; 92(2): 405 - 413. [Abstract] [Full Text] [PDF]
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M. E. Wierman, R. Basson, S. R. Davis, S. Khosla, K. K. Miller, W. Rosner, and N. Santoro Androgen Therapy in Women: An Endocrine Society Clinical Practice Guideline J. Clin. Endocrinol. Metab., October 1, 2006; 91(10): 3697 - 3710. [Abstract] [Full Text] [PDF]
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M. M. Kushnir, A. L. Rockwood, W. L. Roberts, E. G. Pattison, W. E. Owen, A. M. Bunker, and A. W. Meikle Development and Performance Evaluation of a Tandem Mass Spectrometry Assay for 4 Adrenal Steroids Clin. Chem., August 1, 2006; 52(8): 1559 - 1567. [Abstract] [Full Text] [PDF]
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