Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 52: 212-219, 2006. First published November 23, 2005; 10.1373/clinchem.2005.051359
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplements
Right arrow All Versions of this Article:
clinchem.2005.051359v1
52/2/212    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bunk, D. M.
Right arrow Articles by Welch, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bunk, D. M.
Right arrow Articles by Welch, M. J.
Related Collections
Right arrow Laboratory Management
Right arrow General Clinical Chemistry
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2006;52:212-219.)
© 2006 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Characterization of a New Certified Reference Material for Human Cardiac Troponin I

David M. Bunka and Michael J. Welch

1 Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.

aAuthor for correspondence. Fax 301-977-0685; e-mail david.bunk{at}nist.gov.

Background: To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new certified reference material, Standard Reference Material (SRM) 2921: Human Cardiac Troponin Complex.

Methods: The concentration of cTnI in SRM 2921 was determined through a combination of reversed-phase liquid chromatography (LC) with ultraviolet detection and amino acid analysis. Characterization of the intact troponin subunits was accomplished through reversed-phase LC coupled with mass spectrometry. Posttranslational modifications to the cTnI in SRM 2921 were investigated by combining proteolytic digestion with matrix-assisted laser desorption/ionization mass spectrometry. Additionally, reference concentration values for cTnT and cTnC were also determined.

Results: The concentration of human cTnI in SRM 2921 is 31.2 (1.4) mg/L (where 1.4 mg/L is the uncertainty at a 95% level of confidence), as certified through a method that provides traceability to the International System of Units (SI). Reference concentration values of the cTnT and cTnC subunits were determined to be 36.9 (3.8) mg/L and 24.2 (1.3) mg/L, respectively.

Conclusions: This first cTnI reference material should provide SI traceability to clinical cTnI assays once commutability has been validated, and could assist in the international harmonization of cTnI assays as a tool for understanding the underlying causes of interassay variability.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
G. G. Klee
Assay Configuration and Analytic Specificity May Have Major Effects on Prediction of Clinical Outcomes-- Implications for Reference Standards
Clin. Chem., May 1, 2009; 55(5): 848 - 849.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
C.M. Cobbaert, C.W. Weykamp, E.C.H.J. Michielsen, H. Baadenhuijsen, and M.P. van Dieijen-Visser
Time-Dependent Instability of Cardiac Troponins in Human Plasma Spiked with NIST Reference Material 2921
Clin. Chem., December 1, 2008; 54(12): 2078 - 2079.
[Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
V. Zabrouskov, Y. Ge, J. Schwartz, and J. W. Walker
Unraveling Molecular Complexity of Phosphorylated Human Cardiac Troponin I by Top Down Electron Capture Dissociation/Electron Transfer Dissociation Mass Spectrometry
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1838 - 1849.
[Abstract] [Full Text] [PDF]

Home page
 Ann Clin BiochemHome page
D. C Gaze and P. O Collinson
Multiple molecular forms of circulating cardiac troponin: analytical and clinical significance
Ann Clin Biochem, July 1, 2008; 45(4): 349 - 355.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. H. Christenson, S. H. Duh, F. S. Apple, G. S. Bodor, D. M. Bunk, M. Panteghini, M. J. Welch, A. H.B. Wu, S. E. Kahn, and for the American Association for Clinical Chemistr
Toward Standardization of Cardiac Troponin I Measurements Part II: Assessing Commutability of Candidate Reference Materials and Harmonization of Cardiac Troponin I Assays
Clin. Chem., September 1, 2006; 52(9): 1685 - 1692.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. G. Miller, G. L. Myers, and R. Rej
Why commutability matters.
Clin. Chem., April 1, 2006; 52(4): 553 - 554.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Association for Clinical Chemistry.