Clinical Chemistry
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Clinical Chemistry 52: 1820-1824, 2006. First published July 13, 2006; 10.1373/clinchem.2006.070417
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(Clinical Chemistry. 2006;52:1820-1824.)
© 2006 American Association for Clinical Chemistry, Inc.

Technical Briefs

Cell-Free Plasma DNA as a Novel Marker of Aseptic Inflammation Severity Related to Exercise Overtraining

Ioannis G. Fatouros1, Aspasia Destouni2, Konstantinos Margonis1, Athanasios Z. Jamurtas3, Christina Vrettou2, Dimitrios Kouretas4, George Mastorakos5, Asimina Mitrakou6, Kiriakos Taxildaris1, Emmanouel Kanavakis2 and Ioannis Papassotiriou7,a

1 Department of Physical Education and Sports Science, Democritus University of Thrace, Komotini Greece;2 Medical Genetics, Athens University Medical School, Athens, Greece;3 Department of Physical Education & Sports Sciences, University of Thessaly, Trikala, Greece;4 Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece;5 Endocrine Unit, Second Department of Obstetrics and Gynecology, "Aretaieion" Hospital, Athens University Medical School, Athens, Greece;6 Department of Internal Medicine, Henry Dunant Hospital, Athens, Greece;7 Department of Clinical Biochemistry, "Aghia Sophia" Children’s Hospital, Athens, Greece;

aaddress correspondence to this author at: Department of Clinical Biochemistry, "Aghia Sophia" Children’s Hospital, 115 27 Athens, Greece; fax 30-210-7467171, e-mail biochem{at}paidon-agiasofia.gr or jpapasotiriou{at}ath.forthnet.gr


Abstract

Background: Circulating free plasma DNA is implicated in conditions associated with tissue injury, including exercise-induced inflammation, and thus is a potential marker for athletic overtraining.

Methods: We measured free plasma DNA along with C-reactive protein (CRP), creatine kinase (CK), and uric acid (UA) in 17 recreationally trained men participating in a 12-week resistance training regimen (8 resistance multi-joint exercises selected to stress the entire musculature: bench press, squat, leg press, snatch, hang clean, dead lifts, barbell arm curls, and rowing), consisting of 4 training periods (t1, t2, t3, and t4).

Results: Plasma DNA concentrations increased markedly after t1, t2, and t3 and returned to baseline after t4. There were substantial differences between t2 and t1 and between t3 and t2 plasma DNA concentrations. CRP increased by 300% after t2 and by 400% after t3 (there was no difference between t2 and t3 CRP values) compared with baseline (t0). CK increased only after t3. UA increased after t2 and t3, with a greater increase after t3.

Conclusions: This study demonstrates that, after chronic excessive resistance exercise, plasma DNA concentrations increase in proportion to training load, suggesting that plasma DNA may be a sensitive marker for overtraining-induced inflammation.






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