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Clinical Chemistry 54: 525-533, 2008. First published January 17, 2008; 10.1373/clinchem.2007.097386
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Right arrow Clinical Immunology
(Clinical Chemistry. 2008;54:525-533.)
© 2008 American Association for Clinical Chemistry, Inc.

Clinical Immunology

Microarray-Based In Vitro Test System for the Discrimination of Contact Allergens and Irritants: Identification of Potential Marker Genes

Sandra Szameit1,a, Klemens Vierlinger1, Letizia Farmer2, Helga Tuschl2 and Christa Noehammer1

1 Molecular Diagnostics, Austrian Research Centers GmbH, ARC, Seibersdorf, Austria;2 Toxicology, Austrian Research Centers GmbH, ARC, Seibersdorf, Austria.

aAddress correspondence to this author at: Molecular Diagnostics, Austrian Research Centers GmbH, ARC, A-2444 Seibersdorf, Austria; fax +43-50550-3653; e-mail Sandra.Szameit{at}arcs.ac.at.

Background: Animal tests have been used to characterize the potential of chemicals to produce allergic contact dermatitis, but this approach is increasingly a matter of public and political concern. Our aim was to develop and validate an alternative in vitro test that can identify contact allergens.

Methods: We developed a targeted microarray containing oligonucleotide probes for 66 immune-relevant genes and analyzed gene expression in monocyte-derived dendritic cells (Mo-DCs) treated with 1 irritant (SDS) and 2 prominent contact allergens, nickel and Bandrowski’s base (BB), which is the oxidation product of the most important hair dye allergen, p-phenylenediamine.

Results: Comparing RNA amounts in chemical-treated and solvent-treated cells, we identified significant changes in the expression of 21 genes and 10 genes after exposure of immature DCs (iDCs) to nickel and BB, respectively, but not after exposure to SDS. Eight genes were differentially expressed after application of both nickel and BB. Real-time PCR was used to confirm the results for selected genes.

Conclusion: We propose a microarray-based in vitro test that might allow the identification of contact allergens. Independently from donor variability, several immune-relevant genes were up- or downregulated after the application of the tested sensitizers to iDCs, therefore presenting potential marker genes. While reducing the number of laboratory animals used, this test would also enable reliable analysis of chemicals using a human system.






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