Received on October 8, 2003
Accepted on December 19, 2003

Proteomics and Protein Markers

Mass Spectrometric Phenotyping of Val34Leu Polymorphism of Blood Coagulation Factor XIII by Differential Peptide Display

¹ BioVisioN AG, Hannover, Germany
² Institute of Pathology, Medical School Hannover, Hannover, Germany

^* To whom correspondence should be addressed. E-mail: h.tammen{at}biovision-discovery.de.

Background: The Val34Leu mutation in the activation peptide of factor XIII (FXIIIA) correlates with a lower incidence of myocardial infarction and ischemic stroke but an increased risk for hemorrhagic stroke. We describe mass spectrometric detection of the activation peptide variants in human serum.

Methods: We used differential peptide display (DPD) to compare comprehensive peptide maps from pairs of samples (???). Peptides were separated by liquid chromatography, and fractions were subjected to mass spectrometry. Mass spectra of all fractions were combined, giving a peptide map representing a two-dimensional display of peptide masses. After comparison of peptide mass maps, peptides that differentiated FXIIIA phenotypes were identified by mass spectrometry.

Results: Val34Leu polymorphisms of the activation peptide of FXIIIA were identified in ??? serum samples by DPD, and their sequences were confirmed by nanoelectrospray-ionization quadrupole time-of-flight mass spectrometry. Analysis of ??? phenotypes was confirmed by allele-specific genotypic analysis in all (n = ???) volunteers.

Conclusion: DPD provides a simple and easy-to-use phenotype assay with advantages over PCR-based assays in being faster and directly analyzing the compound of interest.