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Received on May 3, 2004
Accepted on September 10, 2004
Clinical Immunology |
1 Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Leipzig, Germany
2 Institute of Clinical Immunology, University Hospital, Leipzig, Germany
3 Department of Paediatrics, St. George's Hospital, Leipzig, Germany
4 Children's Hospital, University of Tübingen, Tübingen, Germany
5 Institute of Immunology, Technical University of Dresden, Dresden, Germany
6 Department of Paediatrics, University of Münster, Münster, Germany
* To whom correspondence should be addressed. E-mail: mothes{at}medizin.uni-leipzig.de.
Background: Celiac disease (CD) is induced by wheat gliadins and related cereal proteins. Anti-gliadin antibodies (AGAs) are present in the serum of CD patients, but these antibodies have lower diagnostic specificity and sensitivity than autoantibodies [anti-endomysium antibodies (AEmAs) and anti-tissue transglutaminase antibodies (AtTGAs)]. Recently, AGAs from CD patients were found to recognize deamidated gliadin peptides, probably formed by the action of tissue transglutaminase.
Methods: We synthesized several gliadin peptides and their glutamine-glutamic acid-substituted counterparts on cellulose membranes and tested their recognition by IgA in sera of 52 AEmA-positive CD patients and 76 AEmA-negative controls in a luminescence assay. For comparison, we assayed IgA concentrations of AGAs, AtTGAs, and AEmAs. For measurement of AtTGAs, we used the human recombinant antigen.
Results: We identified several nonapeptides that were detected with high specificity by IgA in CD patients. Diagnostic accuracy of the peptide antibody assay was highest when peptide PLQPEQPFP was used in combination with peptide PEQLPQFEE within one assay. AGAs were above the cutoff in 14 of the controls, but only 5 of the controls were positive for peptide antibodies. For comparison, 82% and 94% of samples were correctly classified by AGAs and the combination nonapeptide assay, respectively (P = 0.007), and the AtTGAs correctly classified 98%.
Conclusion: The peptide antibody assay has higher diagnostic accuracy than AGAs for distinguishing patients with CD from controls, and has diagnostic accuracy similar to that of AtTGAs.
The following articles in journals at HighWire Press have cited this article:
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  D. Basso, G. Guariso, P. Fogar, A. Meneghel, C.-F. Zambon, F. Navaglia, E. Greco, S. Schiavon, M. Rugge, and M. Plebani Antibodies against Synthetic Deamidated Gliadin Peptides for Celiac Disease Diagnosis and Follow-Up in Children Clin. Chem., January 1, 2009; 55(1): 150 - 157. [Abstract] [Full Text] [PDF]
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 T. Matysiak-Budnik, I. C. Moura, M. Arcos-Fajardo, C. Lebreton, S. Menard, C. Candalh, K. Ben-Khalifa, C. Dugave, H. Tamouza, G. van Niel, et al. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease J. Exp. Med., January 21, 2008; 205(1): 143 - 154. [Abstract] [Full Text] [PDF]
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S. Niveloni, E. Sugai, A. Cabanne, H. Vazquez, J. Argonz, E. Smecuol, M. L. Moreno, F. Nachman, R. Mazure, Z. Kogan, et al. Antibodies against Synthetic Deamidated Gliadin Peptides as Predictors of Celiac Disease: Prospective Assessment in an Adult Population with a High Pretest Probability of Disease Clin. Chem., December 1, 2007; 53(12): 2186 - 2192. [Abstract] [Full Text] [PDF]
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 H. E. Prince Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides Clin. Vaccine Immunol., January 1, 2006; 13(1): 150 - 151. [Abstract] [Full Text] [PDF]
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