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Clinical Chemistry 0: clinchem.2005.051128v1, 2005; 10.1373/clinchem.2005.051128
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Received on April 4, 2005
Accepted on October 4, 2005

Drug Monitoring and Toxicology

Clinical Evaluation of a Lead Mobilization Test Using the Chelating Agent Dimercaptosuccinic Acid

Perrine Hoet 1*, Jean-Pierre Buchet 1, Laurence Decerf 1, Benoît Lavalleye 1, Vincent Haufroid 1, Dominique Lison 1

1 Unit of Industrial Toxicology and Occupational Medicine, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium

* To whom correspondence should be addressed. E-mail: hoet{at}toxi.ucl.ac.be.

Background: The lead mobilization test reflects the mobilizable and likely toxicologically active fraction of the lead body burden. We propose a safe and convenient protocol for this test, to assess concomitant copper and zinc excretion and to determine the size of the chelatable lead pool in nonoccupationally exposed adults.

Methods: The study population included 80 white adults: 40 controls [median blood lead concentration (PbB), 25 µg/L] and 40 lead-exposed individuals (315 µg/L). For all participants, 4- and 24-h baseline urine specimens and a blood sample were obtained, dimercaptosuccinic acid (DMSA) was administered orally (1 g), and additional 4- and 24-h urine specimens were obtained. Determinants of the chelatable urinary lead (DMSA-PbU) were traced by linear regression analysis.

Results: Urinary DMSA and lead excretion peaked within 2-3 h after DMSA administration. The amounts of DMSA, lead, copper, and zinc recovered in the 4-h urinary collections were highly correlated with those in 24-h collections (r = 0.857, 0.859, 0.958, and 0.757, respectively). At PbB concentrations >300 µg/L, the relationship between DMSA-PbU and PbB showed a steep increase and a widespread dispersion of DMSA-PbU around the regression line. After DMSA, copper and zinc excretion rates were increased up to 21- and 7-fold, respectively. No side effects were reported after DMSA.

Conclusions: Determination of DMSA-PbU in a 4-h collection after DMSA is convenient, apparently safe, and inexpensive. An upper reference limit value of 22 µg/4 h is proposed for Belgian reference individuals. The diagnostic value of DMSA-PbU is likely to be contributive for PbB >300 µg/L.




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Copyright © 2005 by the American Association for Clinical Chemistry.