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Received on April 9, 2005
Accepted on September 23, 2005
Proteomics and Protein Markers |
1 Department of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
2 Department of Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, Taiwan
3 Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
4 Division of Hematology/Oncology, Department of Internal Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
5 Department of Otorhinolaryngology, Section of Head and Neck Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan
* To whom correspondence should be addressed. E-mail: ajchen{at}mail.cgu.edu.tw.
Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers in the world. We developed a bead-based affinity-fractionated proteomic method to discover a novel plasma marker for oral cancer.
Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysisTM and ClinProtTM bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database.
Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were <4% and 7%, respectively. Six markers that differentiated between cancer and control spectra were found, with mean (SD) molecular masses of 2664 (1), 2850 (1), 3250 (1), 7735 (2), 7927 (2), and 9240 (2) Da. The 2664-Da marker, identified as a fragment of the fibrinogen 
-chain, had the highest sensitivity (100%) and specificity (97%) for cancer.
Conclusion: The high specificity and sensitivity of the fibrinogen -chain fragment suggest that it may be a clinical useful tumor marker.
The following articles in journals at HighWire Press have cited this article:
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