| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received on July 19, 2006
Accepted on November 28, 2006
Endocrinology and Metabolism |
1 Department of Cardiology and Angiology, Hannover Medical School, Hannover, Germany
2 Department of Gastroenterology, Hepatology Endocrinology, Hannover Medical School, Hannover, Germany
3 Department of Cardiology and Uppsala Clinical Research Center, University of Uppsala, Uppsala, Sweden
* To whom correspondence should be addressed. E-mail: wollert.kai{at}mh-hannover.de.
Background: Growth-differentiation factor 15 (GDF15) is a member of the transforming growth factor 
(TGF-) cytokine superfamily. There has been increasing interest in using circulating GDF15 as a biomarker in patients, for example those with cardiovascular disease.
Methods: We developed an IRMA that uses a polyclonal, affinity chromatography-purified goat antihuman GDF15 IgG antibody, assessed the preanalytic characteristics of GDF15, and determined circulating GDF15 concentrations in 429 apparently healthy elderly individuals and 153 patients with chronic heart failure (CHF).
Results: The assay had a detection limit of 20 ng/L, an intraassay imprecision of 
10.6%, and an interassay imprecision of 12.2%. Specificity was demonstrated with size-exclusion chromatography, parallel measurements with polyclonal and monoclonal anti-GDF15 antibody, and lack of cross-reactivity with TGF-. The assay was not appreciably influenced by the anticoagulant matrix or unrelated biological substances. GDF15 was stable at room temperature for 48 h and resistant to 4 freeze-thaw cycles. Apparently healthy, elderly individuals presented with a median GDF15 concentration of 762 ng/L (25th-75th percentiles, 600-959 ng/L). GDF15 concentrations were associated with age and with cystatin C and C-reactive protein concentrations. CHF patients had increased GDF15 concentrations that were closely related to disease severity.
Conclusion: The IRMA can detect GDF15 in human serum and plasma with excellent sensitivity and specificity. The reference limits and confounding variables defined for apparently healthy elderly individuals and the favorable preanalytic characteristics of GDF15 are expected to facilitate future studies of GDF15 as a biomarker in various disease settings, including CHF.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  L. Lind, L. Wallentin, T. Kempf, H. Tapken, A. Quint, B. Lindahl, S. Olofsson, P. Venge, A. Larsson, J. Hulthe, et al. Growth-differentiation factor-15 is an independent marker of cardiovascular dysfunction and disease in the elderly: results from the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) Study Eur. Heart J., October 1, 2009; 30(19): 2346 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugulle, R. Dechend, F. Herse, M. S. Weedon-Fekjaer, G. M. Johnsen, K. B. Brosnihan, L. Anton, F. C. Luft, K. C. Wollert, T. Kempf, et al. Circulating and Placental Growth-Differentiation Factor 15 in Preeclampsia and in Pregnancy Complicated by Diabetes Mellitus Hypertension, July 1, 2009; 54(1): 106 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Robach, S. Recalcati, D. Girelli, C. Gelfi, N. J. Aachmann-Andersen, J. J. Thomsen, A. M. Norgaard, A. Alberghini, N. Campostrini, A. Castagna, et al. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin Blood, June 25, 2009; 113(26): 6707 - 6715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kempf, J.-M. Sinning, A. Quint, C. Bickel, C. Sinning, P. S. Wild, R. Schnabel, E. Lubos, H. J. Rupprecht, T. Munzel, et al. Growth-Differentiation Factor-15 for Risk Stratification in Patients With Stable and Unstable Coronary Heart Disease: Results From the AtheroGene Study Circ Cardiovasc Genet, June 1, 2009; 2(3): 286 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Eggers, T. Kempf, T. Allhoff, B. Lindahl, L. Wallentin, and K. C. Wollert Growth-differentiation factor-15 for early risk stratification in patients with acute chest pain Eur. Heart J., October 1, 2008; 29(19): 2327 - 2335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nickel, T. Kempf, H. Tapken, J. Tongers, F. Laenger, U. Lehmann, H. Golpon, K. Olsson, M. R. Wilkins, J. S. R. Gibbs, et al. Growth Differentiation Factor-15 in Idiopathic Pulmonary Arterial Hypertension Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 534 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lankeit, T. Kempf, C. Dellas, M. Cuny, H. Tapken, T. Peter, M. Olschewski, S. Konstantinides, and K. C. Wollert Growth Differentiation Factor-15 for Prognostic Assessment of Patients with Acute Pulmonary Embolism Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 1018 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kempf, E. Bjorklund, S. Olofsson, B. Lindahl, T. Allhoff, T. Peter, J. Tongers, K. C. Wollert, and L. Wallentin Growth-differentiation factor-15 improves risk stratification in ST-segment elevation myocardial infarction Eur. Heart J., December 1, 2007; 28(23): 2858 - 2865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Wollert, T. Kempf, B. Lagerqvist, B. Lindahl, S. Olofsson, T. Allhoff, T. Peter, A. Siegbahn, P. Venge, H. Drexler, et al. Growth Differentiation Factor 15 for Risk Stratification and Selection of an Invasive Treatment Strategy in Non ST-Elevation Acute Coronary Syndrome Circulation, October 2, 2007; 116(14): 1540 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kempf, S. von Haehling, T. Peter, T. Allhoff, M. Cicoira, W. Doehner, P. Ponikowski, G. S. Filippatos, P. Rozentryt, H. Drexler, et al. Prognostic Utility of Growth Differentiation Factor-15 in Patients With Chronic Heart Failure J. Am. Coll. Cardiol., September 11, 2007; 50(11): 1054 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.H. Wilson Tang, G. S. Francis, D. A. Morrow, L. K. Newby, C. P. Cannon, R. L. Jesse, A. B. Storrow, R. H. Christenson, COMMITTEE MEMBERS, R. H. Christenson, et al. National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: Clinical Utilization of Cardiac Biomarker Testing in Heart Failure Circulation, July 31, 2007; 116(5): e99 - e109. [Full Text] [PDF]
|
|
|
|
|
|
D. A. Morrow and J. A. de Lemos Benchmarks for the Assessment of Novel Cardiovascular Biomarkers Circulation, February 27, 2007; 115(8): 949 - 952. [Full Text] [PDF]
|
|
|
|
|
|
K. C. Wollert, T. Kempf, T. Peter, S. Olofsson, S. James, N. Johnston, B. Lindahl, R. Horn-Wichmann, G. Brabant, M. L. Simoons, et al. Prognostic Value of Growth-Differentiation Factor-15 in Patients With Non-ST-Elevation Acute Coronary Syndrome Circulation, February 27, 2007; 115(8): 962 - 971. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
