Received on October 27, 2008
Accepted on February 25, 2009

Mini-Review

Steroid Hormone Analysis by Tandem Mass Spectrometry

¹ Department of Medicine, and Department of Pharmacology and General Clinical Research Center, Georgetown University, Washington, DC
² Department of Medicine, Lombardi Department of Oncology, Comprehensive Cancer Center, and Department of Physiology and Biophysics, Georgetown University, Washington, DC

^* To whom correspondence should be addressed. E-mail: sjs44{at}georgetown.edu.

BACKGROUND: New high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays.

CONTENT: We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generation of tandem mass spectrometers has superior limits of quantification, permitting omission of previously employed derivatization steps. The measurement of steroid profiles in the diagnosis and treatment of congenital adrenal hyperplasia, adrenal insufficiency, chronic pelvic pain and prostatitis, oncology (breast cancer), and athletes has important new applications.

CONCLUSIONS: LC-MS/MS now affords the specificity, imprecision, and limits of quantification necessary for the reliable measurement of steroids in human fluids, enhancing diagnostic capabilities, particularly when steroid profiles are available.