| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received on October 20, 2008
Accepted on January 27, 2009
Reviews |
1 Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
2 Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany
3 Centre for Virology, Department of Infection, University College London, London, UK, and Department of Virology, UCL Hospitals NHS Foundation Trust, London, UK
4 Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
5 Centre for Infectious Diseases, University College London, London, UK
6 TATAA Biocenter, Göteborg, Sweden, and Institute of Biotechnology AS CR, Prague, Czech Republic
7 Sequenom, San Diego, California, USA
8 Sigma-Aldrich, Haverhill, UK
9 Physiology Weihenstephan, Technical University Munich, Freising, Germany
10 Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas, USA
11 Department of Pathology, University of Utah, Salt Lake City, Utah, USA, and ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
* To whom correspondence should be addressed. E-mail: s.a.bustin{at}qmul.ac.uk.
BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments.
CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.
SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  C. Yamamizo, S. Kishimoto, and A. Ohmiya Carotenoid composition and carotenogenic gene expression during Ipomoea petal development J. Exp. Bot., November 20, 2009; (2009) erp335v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hammerschmidt, I. Loeffler, and G. Wolf Morg1 heterozygous mice are protected from acute renal ischemia-reperfusion injury Am J Physiol Renal Physiol, November 1, 2009; 297(5): F1273 - F1287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Peleg, G. Baneth, O. Eyal, J. Inbar, and S. Harrus Use of Chimeric DNA-RNA Primers in Quantitative PCR for Detection of Ehrlichia canis and Babesia canis Appl. Envir. Microbiol., October 1, 2009; 75(19): 6393 - 6398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Moreau, E. Voirin, C. Paris, M. Kotera, M. Nothisen, J.-S. Remy, J.-P. Behr, P. Erbacher, and N. Lenne-Samuel Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription Nucleic Acids Res., October 1, 2009; 37(19): e130 - e130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ok, J. P. Latge, C. Baeuerlein, F. Ebel, M. Mezger, M. Topp, O. Kurzai, D. Killian, M. Kapp, G.-U. Grigoleit, et al. Immune Responses of Human Immature Dendritic Cells Can Be Modulated by the Recombinant Aspergillus fumigatus Antigen Aspf1 Clin. Vaccine Immunol., October 1, 2009; 16(10): 1485 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vermeulen, F. Pattyn, K. De Preter, L. Vercruysse, S. Derveaux, P. Mestdagh, S. Lefever, J. Hellemans, F. Speleman, and J. Vandesompele External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data Nucleic Acids Res., September 4, 2009; (2009) gkp721v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Hoffmann, M. Eschbaumer, and M. Beer Real-Time Quantitative Reverse Transcription-PCR Assays Specifically Detecting Bluetongue Virus Serotypes 1, 6, and 8 J. Clin. Microbiol., September 1, 2009; 47(9): 2992 - 2994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mdivani, H. Li, M. Akhalaia, M. Gegia, L. Goginashvili, D. S. Kernodle, G. Khechinashvili, and Y.-W. Tang Monitoring Therapeutic Efficacy by Real-Time Detection of Mycobacterium tuberculosis mRNA in Sputum Clin. Chem., September 1, 2009; 55(9): 1694 - 1700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hosono, M. Kato, K. Kiyotani, T. Mushiroda, S. Takata, H. Sato, H. Amitani, Y. Tsuchiya, K. Yamazaki, T. Tsunoda, et al. CYP2D6 Genotyping for Functional-Gene Dosage Analysis by Allele Copy Number Detection Clin. Chem., August 1, 2009; 55(8): 1546 - 1554. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
