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Review: Ashutosh K. Pathak, Manisha Bhutani, Sachin Kumar, Anant Mohan, and Randeep Guleria Circulating Cell-Free DNA in Plasma/Serum of Lung Cancer Patients as a Potential Screening and Prognostic Tool Clin Chem 2006; 52: 1833-1842 [Abstract] [Full text] [PDF]

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Quantification of circulating DNA by real-time PCR

Oliver Gautschi   (5 February 2007)

Quantification of circulating DNA by real-time PCR 5 February 2007
Oliver Gautschi Send letter to journal: Re: Quantification of circulating DNA by real-time PCR oliver.gautschi{at}ucdmc.ucdavis.edu Oliver Gautschi	Dear Dr. Bruns We studied the review by Pathak et al. (Circulating cell-free DNA in plasma/serum of lung cancer patients as a potential screening and prognostic tool. Clin Chem. 2006 Oct;52(10):1833-42.) with great interest, and we thank the authors for discussing our own work in their review (ref. 65, discussed on text page 4, in table 2 and in table 3). However, we would like to mention that we used GAPDH and not an immunoglobulin heavy chain sequence as the reference gene in our study (Circulating deoxyribonucleic Acid as prognostic marker in non-small-cell lung cancer patients undergoing chemotherapy. Gautschi et al. J Clin Oncol. 2004 Oct 15;22(20):4157-64.). Sincerely, Oliver Gautschi and Annemarie Ziegler Oliver Gautschi, MD, University of California Davis Cancer Center, Sacramento, CA95616, USA Annemarie Ziegler, PhD, University Hospital Zurich, Laboratory of Molecular Oncology, Zurich, CH-8000, Switzerland